600 papers
This study establishes a systematic real-time profiling framework using HiBiT-tagging and split NanoLuc detection to map the temporal translocation kinetics of all 39 Salmonella type III effectors during a 24-hour infection, revealing distinct secretion patterns and a quantitatively resolved functional overlap between T3SS-1 and T3SS-2.
This study demonstrates that while the loss of the apical polar ring component APR9 alone has a moderate effect on *Toxoplasma gondii*, the simultaneous knockout of APR9 and KinesinA paralyzes the parasite by disrupting conoid extension, adhesin secretion, and overall motility, thereby highlighting the critical role of the apical polar ring in regulating multiple subcellular processes essential for invasion and egress.
This study demonstrates that in INS-1 β-cells, MEF2D overexpression impairs mitochondrial respiration, glucose-stimulated insulin secretion, and cell survival, whereas its knockdown enhances these functions, suggesting MEF2D as a potential therapeutic target for diabetes.
This study demonstrates that modulating SPARC expression in mesenchymal stem cells significantly alters their secretome composition, with SPARC overexpression enhancing and SPARC knockdown impairing the regenerative capacity of the secretome to accelerate cutaneous wound healing.
This study reveals that CDK- and mTOR-dependent phosphorylation of the E2 enzyme UBE2H at residues S3/S5 restricts its ubiquitin charging to dynamically regulate CTLH complex activity in response to cell cycle progression and nutrient status, thereby controlling the degradation of specific substrates like NEK9 and AAMP.
This paper presents a deterministic lateral displacement microfluidic device that successfully overcomes the fragility of mature adipocytes to achieve high-purity, high-recovery size-based separation while preserving cell viability and hormonal responsiveness for metabolic research.
This study presents a semi-automated 3D electron microscopy workflow using nnU-Net to segment liver sinusoids and quantify endothelial fenestrations, successfully demonstrating the critical role of BMP9 in fenestration maintenance by comparing wild-type and Bmp9-deleted mice.
This study identifies a lung endothelial niche that regulates human alveolar stem cell fate by using MAPK signaling to sustain self-renewal and, when combined with LATS inhibition, promoting YAP-mediated differentiation into alveolar type 1 cells for effective regeneration in fibrotic lungs.
This study reveals that the molecular aging of ribosomes impairs translational fidelity by increasing pausing and collisions at specific sequences, thereby linking ribosome longevity to altered gene expression during organismal aging.
This study presents the generation of immortalized AT2 cell lines from both healthy and IPF lungs that retain key biological features and form 3D organoids, providing a robust platform for studying alveolar epithelial biology and developing therapies for lung diseases.
This study demonstrates that in *S. pombe*, the synchronous and irreversible onset of anaphase is driven by rapid securin degradation and limited by molecular noise, rather than relying on the positive feedback mechanisms typically associated with major cell cycle transitions.
This study reveals that arrestin-3 acts as a versatile scaffold for multiple MAP3Ks, particularly ZAK, to drive stress-induced JNK3 activation and cell death, and demonstrates that a derived peptide mimicking this scaffolding function can sensitize cancer cells to chemotherapy.
This paper introduces Cellquant, a reproducible, text-based command-line pipeline that automates complex image analysis tasks like segmentation and colocalization for quantitative fluorescence microscopy, enabling biologists to rigorously characterize cellular dynamics such as stress granule formation and nucleolar reorganization without requiring programming expertise.
This study reports the engineering of "Matcha," a bright and thermostable green fluorescent protein derived from Thermal Green Protein via directed evolution in *Sulfolobus acidocaldarius*, which enables live-cell imaging of hyperthermophiles and reveals novel, asymmetric inheritance dynamics of the CdvA division ring during cytokinesis.
This paper introduces photochromic reversion, an imaging modality that enables minute-long single-molecule tracking in living plants, and pairs it with the CASTA machine learning tool to reveal previously inaccessible nanoscale spatial arrest events of plasma membrane proteins.
This study demonstrates that peroxisome dysfunction caused by PEX1 or PEX6 mutations in human retinal pigment epithelium disrupts lipid metabolism and photoreceptor outer segment processing, providing a mechanistic explanation for retinal degeneration in peroxisome biogenesis disorders.
This study presents a high-throughput single-cell proteomics platform that integrates a modified nPOP workflow with IBT16-TMT16 quantitative hyperplexing to achieve deep proteome coverage (up to 3,000 proteins per cell) and ultrahigh throughput (~2,000 cells/day), successfully revealing distinct molecular features in cholangiocarcinoma cells.
This study reveals that Arabidopsis receptor kinase complexes form through a deterministic nanoscale process where accessory receptors maintain a dynamic pool of co-receptors that become spatially arrested upon ligand binding, thereby facilitating efficient signaling without requiring immediate complex activation.
This study demonstrates that Presenilin 1 (PS1) resides in the mitochondrial inner membrane where it regulates cristae junction protein arrangement and cristae formation by preventing ATAD3A oligomerization, thereby maintaining mitochondrial function and preventing dysfunctions associated with Alzheimer's disease.
This study reveals that the continuous degradation of a stem-cell-related translational repression machinery by the Non-stop/dUbcH8/CTLH ubiquitin network safeguards enterocyte identity in young Drosophila, a process that fails during aging due to declining Non-stop levels and Rogue-mediated clearance of the machinery, leading to the reactivation of stem-cell programs and loss of cell identity.